A THROMBIN CLEAVAGE FRAGMENT OF APOLIPOPROTEIN-E EXHIBITS ISOFORM-SPECIFIC NEUROTOXICITY

Citation
Ma. Marques et al., A THROMBIN CLEAVAGE FRAGMENT OF APOLIPOPROTEIN-E EXHIBITS ISOFORM-SPECIFIC NEUROTOXICITY, NeuroReport, 7(15-17), 1996, pp. 2529-2532
Citations number
25
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
09594965
Volume
7
Issue
15-17
Year of publication
1996
Pages
2529 - 2532
Database
ISI
SICI code
0959-4965(1996)7:15-17<2529:ATCFOA>2.0.ZU;2-S
Abstract
A 22 kDa fragment of apoE containing a putative cytotoxi domain was id entified in postmortem human brain tissue and fresh CSF. This fragment is apparently equivalent to the major apoE thrombin cleavage product. In vitro toxicity assays demonstrate that the corresponding fragment derived from recombinantly expressed human apoE is toxic to primary ne urons in culture and that the E4-derived fragment is significantly mor e toxic than the fragment derived from the E3 isoform. These results s uggest that proteolytic fragments of apoE may play a direct role in th e pathology associated with AD and other diseases in which apoE has be en implicated.