RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF THE MAJOR HISTOCOMPATIBILITY COMPLEX-LINKED HEAT-SHOCK PROTEIN-70 (HSP70) GENES OF THE RAT

DNAs from inbred rat strains representing eighteen major histocompatib ility complex (RT1) haplotypes have been tested for restriction fragme nt length polymorphisms of the three heat shock protein 70 (Hsp70) gen es mapping into this complex. The DNAs were digested with restriction enzymes BamHI, EcoRI, PstI or PvuII and assayed by probes hybridizing to all three genes or selectively to Hsp70-1, Hsp70-2 and Hsp70-3, res pectively. Three to four alleles were detected with each enzyme and in total five different strain distribution patterns could be found. Nei ther deletions nor amplifications of genes were detected in the three- gene Hsp70 cluster.