STUDY OF LIPID COMPONENTS OF LUPINUS-MUTABILIS MEAL AND ISOLATES

Citation
F. Millan et al., STUDY OF LIPID COMPONENTS OF LUPINUS-MUTABILIS MEAL AND ISOLATES, Bioscience, biotechnology, and biochemistry, 58(12), 1994, pp. 2258-2260
Citations number
26
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
ISSN journal
09168451
Volume
58
Issue
12
Year of publication
1994
Pages
2258 - 2260
Database
ISI
SICI code
0916-8451(1994)58:12<2258:SOLCOL>2.0.ZU;2-Q
Abstract
Two types of protein isolates have been obtained from defatted Lupinus mutabilis meal. Both, isolate-A and -B, were obtained by alkaline ext raction with 0.2% NaOH, and 0.25% bisulfite, followed by precipitation at the isoelectric point (pH = 4.8). Total lipids were extracted with 86% ethanol, and neutral lipids were separated using a Florisil colum n. The lipids found in the isolates were similar to those found in the original meal, and the following types of compounds were separated, i dentified, and measured: hydrocarbons, waxes, methylesters, triglyceri des, free fatty acids, diglycerides, and free sterols.