PRODUCTION OF ACETYLACETOIN BY BACTERIAL FERMENTATION

Bacillus sp. YUF-4 did not produce acetylacetoin with general culture media, such as bouillon medium containing glucose or acetoin. When dia cetyl was added to a medium cultured for 18-20 h in the presence of gl ucose or acetoin, AAC was produced as culture continued. AAC was assay ed by GLC with a Carbowax 20M capillary column. The AAC produced was p urified by several steps: the final HPLC using a Shodex E411 column wa s effective. The yield of AAC was 346 mg per liter of the medium (7.5% recovery) and the purity was 97%. AAC was identified by H-1-NMR and C -13-NMR.