ENZYMES INVOLVED IN THEOBROMINE PRODUCTION FROM CAFFEINE BY PSEUDOMONAS-PUTIDA NO-352

Authors
ASANO Y KOMEDA T YAMADA H
Citation
Y. Asano et al., ENZYMES INVOLVED IN THEOBROMINE PRODUCTION FROM CAFFEINE BY PSEUDOMONAS-PUTIDA NO-352, Bioscience, biotechnology, and biochemistry, 58(12), 1994, pp. 2303-2304
Citations number
5
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
58
Issue
12
Year of publication
1994
Pages
2303 - 2304
Database
ISI
SICI code
0916-8451(1994)58:12<2303:EIITPF>2.0.ZU;2-9
Abstract
A monooxygenase specific for theobromine demethylation was purified fr om the cell-free extract of Pseudomonas putida No. 352 to homogeneity. Caffeine appears to be demethylated to 7-methylxanthine at least by t wo enzymes, caffeine demethylase and theobromine demethylase. The latt er is sensitive to Zn2+ and possibly caused an accumulation of theobro mine in P. putida No. 352.