I. Dweikat et al., ASSOCIATION OF A DNA MARKER WITH HESSIAN FLY RESISTANCE GENE H9 IN WHEAT, Theoretical and Applied Genetics, 89(7-8), 1994, pp. 964-968
The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat
(Triticum aestivum L.) and genetic resistance has been used effectivel
y over the past 30 years to protect wheat against serious damage by th
e fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25,
have been identified in wheat. With near-isogenic wheat lines differi
ng for the presence of an individual Hessian fly resistance gene, in c
onjunction with random amplified polymorphic DNA (RAPD) analysis and d
enaturing gradient-gel electrophoresis (DGGE), we have identified a DN
A marker associated with the H9 resistance gene. The H9 gene confers r
esistance against biotype L of the Hessian fly, the most virulent biot
ype. The RAPD marker cosegregates with resistance in a segregating F-2
population, remains associated with H9 resistance in a number of diff
erent T. aestivum and T. durum L. genetic backgrounds, and is readily
detected by either DGGE or DNA gel-blot hybridization.