PRODUCTION AND CHARACTERIZATION OF SPECIFIC BOVINE PLASMINOGEN MONOCLONAL-ANTIBODIES

A collection of monoclonal antibodies was raised against bovine plasmi nogen by immunizing mice with purified plasminogen. More than 300 posi tive clones were obtained in 1 fusion experiment (1300 hybrids tested) . Four types of antibodies were characterized through their relative b inding to plasmin and plasminogen in ELISA. About one-third was strict ly specific to plasminogen; the selectivity of this group was confirme d by immunoblots and BIA-core analysis. Cross-reactivity against horse , human, pig, sheep, rabbit, and bovine plasminogens was tested; 41% w ere strictly bovine specific.