A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed
in Brassica napus pistils, was isolated by differential screening. PC
R was used to complete the Pis 63 sequence (Pis 63-1) and to obtain th
e sequence of another related cDNA (Pis 63-2). Northern blot and in si
tu analyses demonstrated that these transcripts are expressed in the s
tigma throughout flower development. Pis 63-1 and Pis 63-2 display sim
ilarity to a cotton fibre cDNA clone.