COMPARATIVE-EVALUATION OF VARIOUS SEROLOGICAL TESTS FOR DETECTION OF AVIAN ADENOVIRUSES IN CHICKEN TISSUES

Tissue samples (75) from cases of inclusion body hepatitis (IBH) and r espiratory infections were subjected to agar-gel precipitation test (A GPT), counterimmunoelectrophoresis (CIE) and double antibody sandwich ELISA for direct detection of avian adenovirus (AAV). In AGPT, CHE and ELISA, respectively, 9, 20 and 31 samples were positive. Isolation in chicken embryo liver (CEL) cell culture revealed 17 isolates from IBH cases and 15 from respiratory infections. The identification of isola tes was done on the basis of characteristic cytopathic effects (CPE), including demonstration of basophillic intranuclear (I/N) inclusion bo dies and serological tests, viz. AGPT, CIE and ELISA. ELISA was the mo st sensitive test as it detected all the 32 isolates as compared to CI E (30) and AGPT (14).