BACTERIOPHAGE-TYPING OF STAPHYLOCOCCUS-AUREUS OF BOVINE MASTITIS ORIGIN

Fifty strains of coagulase-positive Staphylococcus aureus, procured fr om milk samples of cows and buffaloes and with a history of clinical a nd subclinical cases of mastitis, were subjected to bacteriophage typi ng using international basic set as well as basic bovine set of phages . Phage typing with the international basic human set showed that 10 ( 20%) strains were typeable. With the basic bovine set of phages 17 (34 %) were typeable. Of the 50 strains typed with the international basic set, 1 (2%) belonged to group 1/3NA, 2 (4%) to group 3, 6 (12%) to gr oup 3/NA and 1 (2%) to group NA. Based on the analysis of results usin g basic bovine set of phages the distribution of the 17 typeable strai ns out of the total 50 strains was 1 (2%) in group 2, 3 (6%) in group 3, 4 (8%) in group 4, 1 (2%) each in groups 3 and 4, 1 (2%) each in gr oup 3, 1 and miscellaneous, 7 (14%) in group 4, and miscellaneous in o rder of frequency. Analysis of the 50 isolates of Staph. aureus with i nternational basic set of phages and basic set of phages revealed 8 ph age lytic patterns.