A. Leonard et al., DOSE-EFFECT RELATIONSHIP FOR IN-VIVO AND IN-VITRO INDUCTION OF DICENTRIC ABERRATIONS IN BLOOD-LYMPHOCYTES OF CHILDREN, Radiation research, 141(1), 1995, pp. 95-98
Chromosome aberrations induced in vivo were studied in nine children 5
-12 years old treated with total-body high-energy photon irradiation (
pulsed exposure from a LINAC) for different types of malignant disease
s. Dose-effect relationships were obtained for each child by taking br
ood at different times during exposure. In vitro dose-effect relations
hips for chromosome aberrations in children and adults were obtained b
y exposing blood under the same conditions as the children. Exposure i
n vivo and in vitro yielded similar linear-quadratic dose-effect relat
ionships for dicentric aberrations. The response in vitro was slightly
greater than in vivo, but the difference was not very large. It is co
ncluded that the dose-effect relationship for dicentric chromosome abe
rrations obtained in vitro for adults can be used for biological dosim
etry in irradiated children. Some of the children displayed a high num
ber of ''rogue cells'' before exposure; this may be due to the maligna
nt disease as it was not found in the healthy controls.