I. Ieiri et al., PHARMACOKINETIC STUDY OF ZONISAMIDE IN PATIENTS UNDERGOING BRAIN SURGERY, Journal of Pharmacy and Pharmacology, 48(12), 1996, pp. 1270-1275
To test whether the concentration of the anticonvulsant zonisamide in
erythrocytes reflects the brain concentration and the clinical respons
e of the drug, its pharmacokinetics were studied in nine patients unde
rgoing surgery for brain tumour. Erythrocyte, total, and free serum co
ncentrations in samples drawn on the day of brain surgery were compare
d with levels on a day after the operation. In three patients zonisami
de and its major metabolite, 2-sulphamoylacetylphenol, were also analy
sed in urine. The area under the curve of the free and the erythrocyte
concentration did not differ between the two study phases whereas the
area under the curve of the total serum concentration was significant
ly lower on the day of the operation, and this was associated with sig
nificant increases in total clearance (15.4 compared with 12.7 mL kg(-
1) h(-1), P < 0.05, n = 9) and renal clearance (5.4 compared with 3.3
ml kg(-1) h(-1), P < 0.05, n = 3), and non-significant change in nonre
nal clearance (7.7 on the day of operation compared with 8.4 ml kg(-1)
h(-1) on the post-operation day, n = 3). Zonisamide distribution was
also altered by the operative procedure, as evidenced by a higher volu
me of distribution (1.48 compared with 0.87 L kg(-1), P < 0.05, n = 9)
. The binding of zonisamide was characterized on both days. Zonisamide
binding to erythrocytes seemed to occur by two processes: a saturable
process and a non-saturable linear process. The maximum binding capac
ity to erythrocytes (31.6 vs 29.7 mu g mL(-1)) did not differ on the t
wo days; however, increases in the dissociation binding constant (+28%
) and the proportionality constant (+24%) were observed on the day of
the operation, suggesting that the zonisamide concentration in erythro
cytes was greater on the day of the operation. Brain surgery appears t
o be one of the possible factors altering the rate of elimination of z
onisamide and the uptake of the drug by erythrocytes.