PREPARATION AND PROTEIN CONJUGATION OF A DIVINYL SULFONE DERIVATIZED BIFUNCTIONAL CHELATING AGENT

A new bifunctional chelating agent with a novel linking arm, benzyl)}- 1,3-propane-diamine-N,N,N',N'-tetraacetic acid (VS-PDTA) was synthesiz ed and was conjugated to protein for the purpose of attaching radiomet als to monoclonal antibodies (MAbs). The effect of various parameters such as ligand concentration, protein concentration, pH, temperature a nd reaction period on the conjugation have been examined using chromat ographic (SE and TLC) analysis after labeling with In-111. The paramet ers and chemical variables studied have significant effects on the eff iciency and rate of protein conjugation.