ADAPTATION OF THE MTT (3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYL TETRAZOLIUM BROMIDE) ASSAY FOR THE DETERMINATION OF VIRUS-NEUTRALIZING ANTIBODIES USING THE VIRUS-NEUTRALIZATION ASSAY

Authors
HADDAD EE WHITFILL CE RICKS CA FREDERICKSEN T ROWE D OWEN L BALDRIDGE A MURRAY L THOMA JA
Citation
Ee. Haddad et al., ADAPTATION OF THE MTT (3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYL TETRAZOLIUM BROMIDE) ASSAY FOR THE DETERMINATION OF VIRUS-NEUTRALIZING ANTIBODIES USING THE VIRUS-NEUTRALIZATION ASSAY, Avian diseases, 38(4), 1994, pp. 755-761
Citations number
11
Categorie Soggetti
Veterinary Sciences
Journal title
Avian diseases → ACNP
ISSN journal
00052086
Volume
38
Issue
4
Year of publication
1994
Pages
755 - 761
Database
ISI
SICI code
0005-2086(1994)38:4<755:AOTM(T>2.0.ZU;2-O
Abstract
The tetrazolium salt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl te trazolium bromide] has been widely used for bioassays. Herein is descr ibed the use of the MTT dye with a virus-neutralization (VN) assay to titer infectious bursal disease virus (IBDV)-neutralizing antibodies. A standard VN assay using chicken embryo fibroblasts (CEFs) and IBDV w as used for the assessment of IBDV-neutralizing antibodies. The percen t of CEF killing due to IBDV was quantitated using MTT, and the absorb ance (A) data were used to calculate the VN antibody titer. This metho d of calculation offers the expression of VN titer in terms of units o f activity per unit of volume.