ASSOCIATION BETWEEN A CYTOCHROME-P450 CYPIA1 GENOTYPE AND INCIDENCE OF LUNG-CANCER

We analysed 267 samples from patients with lung cancer and 54 samples from patients with pancreatic cancer and classified them into three ty pes (A, B and C) based on their P450CYPIA1 genotypes. This was perform ed by restriction fragment length polymorphism (RFLP) analysis of DNA (that had been amplified) using the polymerase chain reaction (PCR). O ur results reconfirmed previous studies with RFLP analyses by revealin g significantly higher frequencies of C type in lung cancer patients, particularly light smokers with squamous cell carcinoma (p < 0.001). A lso when we compared the appearance rate of P450CYPIA1 genotype in lun g cancer patients between metastasis group and no metastasis group, ly mph node or distant metastasis was more frequently observed among the patients of squamous cell carcinoma, especially the light smoking grou p, with C genotypes (p < 0.05). These results suggested that this geno type is not only associated with the etiology of lung cancer but also with the status of metastasis, Furthermore, genotyping could be useful in predicting the incidence of lung cancer in the smokers and the gen eral population as well as the prognosis of individuals who develop th is disease.