A 26-KDA OUTER-MEMBRANE PROTEIN, OMPK, COMMON TO VIBRIO SPECIES IS THE RECEPTOR FOR A BROAD-HOST-RANGE VIBRIOPHAGE, KVP40

Authors
INOUE T MATSUZAKI S TANAKA S
Citation
T. Inoue et al., A 26-KDA OUTER-MEMBRANE PROTEIN, OMPK, COMMON TO VIBRIO SPECIES IS THE RECEPTOR FOR A BROAD-HOST-RANGE VIBRIOPHAGE, KVP40, FEMS microbiology letters, 125(1), 1995, pp. 101-105
Citations number
13
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
125
Issue
1
Year of publication
1995
Pages
101 - 105
Database
ISI
SICI code
0378-1097(1995)125:1<101:A2OPOC>2.0.ZU;2-T
Abstract
KVP40 is a broad-host-range vibriophage forming plaques on strains of at least eight Vibrio and one Photobacterium species. A spontaneous KV P40-resistant mutant, R4000, derived from Vibrio parahaemolyticus 1010 lacked a 26-kDa outer membrane protein designated OmpK. KVP40 was ina ctivated by outer membrane and OmpK prepared from 1010, but not by out er membrane from R4000. These results strongly suggest that OmpK is th e receptor for KVP40. Immunoblotting analyses using an anti-OmpK rabbi t serum revealed that OmpK or its homologs of molecular masses 25-29 k Da were distributed widely among Vibrio and Photobacterium strains inc luding those naturally resistant to KVP40.