DIFFERENTIAL INDUCTION OF GENES RELATED TO SOLVENT FORMATION DURING THE SHIFT FROM ACIDOGENESIS TO SOLVENTOGENESIS IN CONTINUOUS-CULTURE OFCLOSTRIDIUM-ACETOBUTYLICUM
U. Sauer et P. Durre, DIFFERENTIAL INDUCTION OF GENES RELATED TO SOLVENT FORMATION DURING THE SHIFT FROM ACIDOGENESIS TO SOLVENTOGENESIS IN CONTINUOUS-CULTURE OFCLOSTRIDIUM-ACETOBUTYLICUM, FEMS microbiology letters, 125(1), 1995, pp. 115-120
The expression of all sequenced acetone and butanol formation genes wa
s followed using mRNA analysis during the shift from acidogenesis to s
olventogenesis in continuous culture of Clostridium acetobutylicum. Di
fferential induction or derepression of the bdhA, bdhB, and ade genes
as well as the sol operon was observed during the pH-induced shift. Th
e order of induction of the three different butanol dehydrogenase gene
s was found to be bdhA-sol operon-bdhB, offering an explanation for th
e physiological role of the respective enzymes. Peak mRNA synthesis of
an autolysin and a heat shock gene at the onset of solventogenesis wa
s detected in addition to the above-mentioned genes. None of the hithe
rto sequenced genes of butanologenic enzymes was found to be involved
in butanol production during the Methyl viologen-induced shift, indica
ting the presence of yet unknown genes encoding alcohol and aldehyde d
ehydrogenases.