ELECTROREDUCTION OF THE SCHIFF-BASES FROM PYRIDOXAL-5'-P, PYRIDOXAL AND L-ISOLEUCINE - INFLUENCE OF THE AMINO-ACID MOIETY ON THE SCHIFF-BASES DERIVED FROM ALPHA-AMINO-ACIDS

The electrochemical behaviour of the Schiff bases derived from pyridox al-5'-P (PLP) and pyridoxal (PL) with L-isoleucine (Ile) has been stud ied in buffered aqueous solution. The formation of the Schiff base, it s dependence on the pH and the electroreduction scheme at different pH values have been compared to those of the Schiff bases derived from L -alanine (Ala) and L-leucine (Leu). The observed differences seem to b e produced by a hydrophobic effect of the amino acid moiety. This find ing can be helpful for understanding the coenzyme environment on the p rotein binding site.