FUNCTIONS OF HISTONE-LIKE PROTEINS IN TIME INITIATION OF DNA-REPLICATION AT ORIC OF ESCHERICHIA-COLI

Authors
ROTH A URMONEIT B MESSER W
Citation
A. Roth et al., FUNCTIONS OF HISTONE-LIKE PROTEINS IN TIME INITIATION OF DNA-REPLICATION AT ORIC OF ESCHERICHIA-COLI, Biochimie, 76(10-11), 1994, pp. 917-923
Citations number
46
Categorie Soggetti
Biology
Journal title
Biochimie → ACNP
ISSN journal
03009084
Volume
76
Issue
10-11
Year of publication
1994
Pages
917 - 923
Database
ISI
SICI code
0300-9084(1994)76:10-11<917:FOHPIT>2.0.ZU;2-P
Abstract
Using methidiumpropyl-EDTA (MPE) footprinting we found one specific bi nding site for FIS protein in the E coli replication origin, oriC. We mutagenized the binding sites for FIS and IHF in oriC and analyzed the effect of the mutations on protein binding and oriC function. The rep lication efficiency of oriC plasmids paralleled the ability of the mut ated DNA fragments to bind IHF or FIS. We conclude that these histone- like proteins function in cis in the initiation of DNA replication at oriC.