AVRXA10 PROTEIN IS IN THE CYTOPLASM OF XANTHOMONAS-ORYZAE PV ORYZAE

Authors
YOUNG SA WHITE FF HOPKINS CM LEACH JE
Citation
Sa. Young et al., AVRXA10 PROTEIN IS IN THE CYTOPLASM OF XANTHOMONAS-ORYZAE PV ORYZAE, Molecular plant-microbe interactions, 7(6), 1994, pp. 799-804
Citations number
26
Categorie Soggetti
Plant Sciences","Biothechnology & Applied Migrobiology
Journal title
Molecular plant-microbe interactions → ACNP
ISSN journal
08940282
Volume
7
Issue
6
Year of publication
1994
Pages
799 - 804
Database
ISI
SICI code
0894-0282(1994)7:6<799:APIITC>2.0.ZU;2-K
Abstract
AVRXa10 from Xanthomonas oryzae pv. oryzae was tagged with a unique hy drophilic octapeptide (FLAG) to permit antibody-mediated identificatio n and purification of the gene product. X. o. pv. oryzae that produced tagged AVRXa10 elicited a hypersensitive response (HR) on rice cultiv ars containing the resistance gene Xa-10, but not on cultivars lacking Xa-10. The tagged AVRXa10 protein purified from Escherichia coli or X . o. pv. oryzae did not elicit a hypersensitive response in rice with the Xa-10 resistance gene. Anti-FLAG monoclonal antibodies reacted wit h a 119-kDa protein in both E. coli and X. o. pv. oryzae cells express ing the tagged avrXa10 gene. Polyclonal antibodies raised against puri fied AVRXa10 protein reacted with the 119-kDa protein and several addi tional proteins from X. o. pv. oryzae, which probably are the products of genes related to avrXa10. Biochemical fractionation and immunoelec tronmicroscopy analysis was used to demonstrate that AVRXa10 was locat ed in the cytoplasm of X. o. pv. oryzae cells when grown in planta or in culture medium.