N,N-DIETHYLAMINO)-N-OCTYL-3,4,5-TRIMETHOXYBENZOATE ACTIONS ON CALCIUMDYNAMICS IN CULTURED VASCULAR SMOOTH-MUSCLE CELLS

AIM: To study 8-(N, N-Diethylamino)-n-octyl-3,4,5-trimethoxybenzoate ( TMB), a potent Ca2+-antagonist, actions on cellular calcium dynamics i n vascular smooth muscle cell (VSMC) cultures. METHODS: A7r5 VSMC were cultured with Fura-2 measurements of intracellular Ca2+ concentration , [Ca2+](i). RESULTS: TMB reduced [Ca2+](i) from control levels and bl ocked [Ca2+](i) increase caused by norepinephrine (NE) and 2,5-di(t-bu tyl)-1,4-benzohydroquinone (BHQ). [Ca2+](i) reduction by TMB was furth er enhanced by ryanodine. CONCLUSION: TMB is an effective agent for bl ocking the [Ca2+](i) increase caused by NE and BHQ and for enhancing t he [Ca2+](i) reduction caused by ryanodine.