K. Janeczko, THE PROLIFERATIVE ACTIVITY OF ASTROCYTES AFTER IMMUNOGLOBULIN-G UPTAKE IN THE INJURED MOUSE CEREBRAL HEMISPHERE, Folia histochemica et cytobiologica, 32(4), 1994, pp. 239-241
Dividing cells were labeled with [H-3]thymidine injected at different
intervals following the injury of the rat cerebral hemisphere. Brain s
ections were double immunostained for GFAP and for immunoglobulin G (I
gG), and subjected to autoradiography. Thereafter, three cell types we
re counted within the injury area: (1) autoradiographically labeled an
d (2) unlabeled astrocytes immunopositive both for GFAP and IgG (GFAP/IgG-), and (3) autoradiographically labeled astrocytes immunopositive
exclusively for GFAP (GFAP+/IgG-). Reactive proliferation of GFAP+/Ig
G- astrocytes began on the Ist day after injury and reached its maxima
l intensity on day 4, whereas the population of non-proliferating GFAP
+/IgG+ astrocytes increased continuously during 8 days after injury an
d then decreased. Proliferating GFAP+/IgG+ astrocytes were found only
occasionally during the whole examined period and did not-display sign
ificant quantitative changes. The results suggest that the ability of
astrocytes to proliferate after IgG uptake is very low in comparison w
ith those containing no IgG.