Amm. Dasilva et al., VIABILITY AND MORPHOLOGICAL ALTERATIONS OF PARACOCCIDIOIDES-BRASILIENSIS STRAINS PRESERVED UNDER MINERAL-OIL FOR LONG PERIODS OF TIME, Mycoses, 37(5-6), 1994, pp. 165-169
We evaluated the survival and the morphological alteration of 70 strai
ns of Para-coccidioides brasiliensis maintained in the Fungal Culture
Collection of Institute Oswaldo Cruz and initially preserved by succes
sive subculturing and later under mineral oil at room temperature from
1923 to 1992. Of the 70 strains preserved under mineral oil, 18 (26%)
continued to be viable. The mycelia of the 18 viable strains and the
mycelia of four representatives of the non-viable strains presented tr
ansitional micromorphology under mineral oil. Macroscopy and microscop
y of the first subcultures of five of the seven strains, taken as rand
om samples from the 18 viable strains and plated onto peptone-yeast ex
tract-glucose agar, glucose-glycine-yeast extract agar and brain-heart
infusion agar media at room temperature, revealed that all samples ap
peared to be in a transition phase (Y reversible arrow M). These five
strains were unable to grow and complete thermal dimorphism at a tempe
rature of 37 degrees C. Only two strains were able to complete the ent
ire dimorphic process. The period of preservation of these strains und
er oil was relatively short, i.e. 9 and 10 years. The results demonstr
ate that less widely spaced subculturing and more appropriate culture
and environmental conditions are needed to preserve P. brasiliensis st
rains under oil.