Yc. Liu et al., DISCORDANT IMMUNOPHENOTYPE OF CHRONIC B-CELL LYMPHOPROLIFERATIVE DISORDERS IN SIMULTANEOUS SPECIMENS FROM BONE-MARROW AND PERIPHERAL SITES, Archives of pathology and laboratory medicine, 119(1), 1995, pp. 53-58
Citations number
25
Categorie Soggetti
Pathology,"Medical Laboratory Technology","Medicine, Research & Experimental
This study consisted of 10 cases of chronic B-cell lymphoproliferative
disorders that had simultaneous specimens obtained from both bone mar
row and peripheral sites for flow cytometric immunophenotyping. The im
munophenotyping results of peripheral sites from all 10 cases showed a
monoclonal B-cell proliferation expressing monoclonal surface immunog
lobulin, CD19, CD20, HLA-DR, and CD5 (except 1 case). Eight (80%) of t
he 10 cases, however, demonstrated discordant immunophenotypes with my
eloid-associated marker expression (CD13, CD11b, and/or CD15) found on
ly in the bone marrow. Patients with CD13 or CD11b marker expression i
n the bone marrow followed an aggressive clinical course with advanced
Rai's stage and a diffuse or mixed bone marrow infiltration pattern o
r disease transformation. These results indicate that discordant immun
ophenotypes of malignant cells from different body sites occur in chro
nic B-cell lymphoproliferative disorders and are not uncommon. Additio
nally, myeloid-associated markers, which some investigators have descr
ibed as being associated with an unfavorable clinical course, may be e
xpressed only in bone marrow specimens in these disorders. Thus, bone
marrow specimens may be preferential in determining myeloid-associated
marker expression in chronic B-cell lymphoproliferative disorders.