L. Vian et al., EVALUATION OF HYDROQUINONE AND CHLORAL HYDRATE ON THE IN-VITRO MICRONUCLEUS TEST ON ISOLATED LYMPHOCYTES, Mutation research. Section on environmental mutagenesis and related subjects, 334(1), 1995, pp. 1-7
The cytochalasin B micronucleus test was performed in human peripheral
lymphocyte cultures to assay the ability of hydroquinone and chloral
hydrate to induce micronuclei, in the presence or absence of an exogen
ous metabolic activation system. Cultures and readings were performed
in duplicate. No significant damage Was found after treatment with chl
oral hydrate in this test system, whereas hydroquinone induced a clear
positive response in one culture in the presence of metabolic activat
ion during the G1 phase. Isolated lymphocytes used as a test system pr
ovide information about the test compound itself without interference
by blood components. Comparison of the two readers' data showed few ma
rked discrepancies in the number of micronuclei recorded in binucleate
d cells. Strict criteria for data analysis are therefore necessary to
avoid intra-assay or operator variability.