ESTROGEN-RECEPTOR MESSENGER-RNA EXPRESSION IN HUMAN BENIGN PROSTATIC HYPERPLASIA - DETECTION, LOCALIZATION, AND MODULATION WITH A LONG-ACTING GONADOTROPIN-RELEASING-HORMONE AGONIST

We have analyzed human benign prostatic hyperplastic (BPH) tissue deri ved from eight radical prostatectomy specimens from patients with pros tate cancer for the expression of the estrogen receptor (ER) messenger RNA. Four of the eight patients received a long-acting gonadotropin-r eleasing hormone agonist (GnRHa) for 4 months prior to surgery. An RNa se protection assay utilizing six riboprobes spanning most of the ER p rotein-coding sequences demonstrated expression of the ER mRNA in huma n BPH tissue. A comparison of ER mRNA expression in four patients who had received 4 months pretreatment with the GnRHa vs. the four untreat ed patients suggested that there is upregulation of ER mRNA expression with the GnRHa treatment. The combined techniques of in situ hybridiz ation and immunocytochemistry localized the ER mRNA expression to the prostatic basal epithelial cells and stroma. We conclude that ER mRNA is expressed in human BPH tissue and that this expression is modulated by treatment with a long-acting GnRH agonist.