MULTI-PRINS - MULTIPLE SEQUENTIAL OLIGONUCLEOTIDE PRIMED IN-SITU DNA-SYNTHESIS REACTIONS LABEL SPECIFIC CHROMOSOMES AND PRODUCE BANDS

Authors
SPEEL EJM LAWSON D HOPMAN AHN GOSDEN J
Citation
Ejm. Speel et al., MULTI-PRINS - MULTIPLE SEQUENTIAL OLIGONUCLEOTIDE PRIMED IN-SITU DNA-SYNTHESIS REACTIONS LABEL SPECIFIC CHROMOSOMES AND PRODUCE BANDS, Human genetics, 95(1), 1995, pp. 29-33
Citations number
9
Categorie Soggetti
Genetics & Heredity
Journal title
Human genetics → ACNP
ISSN journal
03406717
Volume
95
Issue
1
Year of publication
1995
Pages
29 - 33
Database
ISI
SICI code
0340-6717(1995)95:1<29:M-MSOP>2.0.ZU;2-M
Abstract
A fast method for identifying several chromosomes with chromosome-spec ific oligonucleotide primers directing an in situ labeling reaction is described. Up to three reactions distinguished by different fluorochr omes (fluorescein isothiocyanate, rhodamine/Texas red, p-amino-methyl- cyclohexane carboxylic acid) can currently be performed. Prospects for increasing this up to seven colors, and for the future of the process in prenatal diagnosis are discussed.