SUBSTANCE-P BINDING IN NORMAL NEONATAL FORESKIN

The distribution of binding sites for NTE-biotinyl-[Arg3]-substance P (SPB) was demonstrated in neonatal. foreskin using a conjugate of stre ptavidin with horseradish peroxidase. The observed binding is reversib le, and may be abrogated by either the non-peptide substance P recepto r antagonist, CP-96,345, or by unlabelled substance P. The generalized epidermal distribution and focal dermal localization of SPB binding s uggest that although NK-1 receptors are abundant in human neonatal for eskin, neuromodulatory mechanisms may play a significant role in epide rmal physiology.