G. Bouche et al., ACTIVATION OF RDNA TRANSCRIPTION BY FGF-2 - KEY ROLE OF PROTEIN-KINASE CKII, Cellular & molecular biology research, 40(5-6), 1994, pp. 547-554
Basic Fibroblast Growth Factor-2 (FGF-2) promotes G1 to S transition o
f quiescent sparse adult bovine aortic endothelial cells. In addition
to signal transduction through interaction with tyrosine kinase high a
ffinity receptor, FGF-2 is translocated to the nucleus and accumulated
into the nucleolus. These data suggest that FGF-2 functions directly
in nuclear events. In vivo, correlations were established between the
entrance of FGF-2 into the nucleus and an increase in rDNA transcripti
on and in protein phosphorylation. In vitro, in experiments carried ou
t with nuclei isolated from quiescent cells, addition of FGF-2 increas
es rDNA transcription by a factor of 5 and also increases protein phos
phorylation. Nucleolin, a factor involved in control of rDNA transcrip
tion is preferentially phosphorylated. It has been shown that nucleoli
n and other factors implicated in rDNA transcription are substrates of
protein kinase CKII. Using purified kinase CKII and nucleolin in an i
n vitro phosphorylation assay, we have shown that FGF-2 activates the
protein kinase activity. These results suggest that FGF-2 could act as
an activator of rDNA transcription through interactions with the prot
ein kinase CKII.