A. Quaroni et J. Hochman, DEVELOPMENT OF INTESTINAL-CELL CULTURE MODELS FOR DRUG TRANSPORT AND METABOLISM STUDIES, Advanced drug delivery reviews, 22(1-2), 1996, pp. 3-52
Cell culture models offer many advantageous features for the analysis
of drug transport and drug metabolism. From a basic research perspecti
ve, these systems offer the potential for manipulating the environment
or cellular properties as a means to address mechanistic questions. F
rom a drug discovery perspective, cell culture models can be used to e
xpedite identification of compounds with favorable pharmacokinetic pro
perties, and to evaluate structure-absorption/metabolism relationships
. In this review, we will use the intestinal epithelium as an example
for discussing issues associated with the development of new cell cult
ure models for evaluating drug metabolism. Specifically, we will discu
ss biological properties of the intestinal epithelium and address biol
ogical and practical consideration in the application of tumor cell li
nes, short-term primary cultures, stem-like cell cultures, and oncogen
e immortalized cells as approaches to establishing models for the inte
stinal epithelium.