A SIMPLIFIED METHOD FOR THE ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM ASPERGILLUS-NIDULANS

We have developed a simple method for obtaining DNA from mycelium of t he fungus Aspergillus nidulans. A single isopropanol preparation yield s good quality high molecular weight DNA preparations that are not con taminated with proteins or salts, and that are easy to solubilize and to digest with restriction enzymes. High yields (approximately 1.6 mg DNA per gram of wet mycelium) are obtained. Contamination with RNA is minimal and there is no need to use RNase. It has been successfully us ed in our laboratory for many molecular biology experiments.