SENSITIVE COLORIMETRIC CYTOTOXICITY MEASUREMENT USING ALARMAR BLUE

Authors
Citation
B. Page et M. Page, SENSITIVE COLORIMETRIC CYTOTOXICITY MEASUREMENT USING ALARMAR BLUE, Oncology Reports, 2(1), 1995, pp. 59-61
Citations number
9
Categorie Soggetti
Oncology
Journal title
ISSN journal
1021335X
Volume
2
Issue
1
Year of publication
1995
Pages
59 - 61
Database
ISI
SICI code
1021-335X(1995)2:1<59:SCCMUA>2.0.ZU;2-4
Abstract
Cytotoxicity testing of anticancer drugs requires techniques which are sensitive, reproductible and applicable to large scale testing using automated instruments. End point staining of cells or viability stains are reliable but they are often not sensitive enough or not suitable for automation. We have already described a fluorometric cytotoxicity assay using a non fluorescent substrate, Alarmar Blue, which upon redu ction in living cells, yields a fluorescent product. This assay is sen sitive and highly reproductible but it is limited since automated fluo rescent plate readers are not found frequently in laboratories. The co nditions are now described for using Alamar Blue as a substrate in a c olorimetric cytotoxicity assay. This new economical assay could be use d with advantage for large scale screening of cytotoxic agents in micr otitration plates.