MICROBIOSENSOR BASED ON AN INTEGRATED THERMOPILE

A microbiosensor based on an integrated thermopile was designed and fa bricated on a quartz chip. The thermopile, which was manufactured by d oping boron in polysilicon together with aluminium, provided a potenti al output of ca. 2 mV/K. A silicone rubber membrane was used to form a nd seal the microchannel. The total column volume was 20 mu l. Glucose oxidase and catalase were co-immobilized on spherical CPG beads (cont rolled pore glass) and in turn charged into the microchannel. Using 1 mu l sample volumes, a linear range of 2 to 25 mM glucose was obtained using a flow rate of 105 mu l/min. The relative standard deviation fo r 100 glucose samples (10 mM) was 5%.