Pz. Liu et al., POLYACRYLAMIDE-MODIFIED POLYPROPYLENE HOLLOW FIBERS FOR CAPILLARY ELECTROPHORESIS, The Journal of microcolumn separations, 6(6), 1994, pp. 581-589
Surface treatment methods were developed for bonding of a polyacrylami
de layer on the inside surface of polypropylene hollow fiber columns f
or capillary electrophoresis. Details of different modification proced
ures involving surface activation and in situ polymerization, using dy
namic and static coating of reagents, are described. Both linear and c
ross-linked polyacrylamide modified surfaces greatly improved the elec
trophoretic performance of the capillaries. The column efficiencies fo
r model proteins reached as high as 370,000 theoretical plates on an 8
5 mu m i.d. polyacrylamide surface-treated polypropylene hollow fiber
column. A variety of applications, including the separation of several
very polar compounds at a pH of 10.6, were demonstrated. Reproducibil
ity measurements were used to illustrate the performance and high pH s
tability of the resultant columns.