EFFECTS OF HYPOTHERMIA ON THE SURVIVAL AND CRYOPRESERVATION OF MINIPIG ILEAL CELLS AND CHINESE-HAMSTER OVARY CELLS

Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypo thermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 3 7 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines e ven though epithelioid IPI-2I cells were more tolerant to hypothermia than IPI-1 fibroblasts. Epithelioid cells survived 3 days at 25 degree s C with unaltered expression of cytokeratin-18 whereas colonies of fi broblasts did not survive more than a day at 25 degrees C (p < 0.001). These results suggest that hypothermia-tolerance of pig ileal cell li nes might differ according to cell lineage calling for further experim ents on small intestinal primary cell culture.