CONSTRUCTION AND PRELIMINARY-ANALYSIS OF THE ICRF HUMAN P1 LIBRARY

P1 clone libraries have now been established as effective complements to cosmid and yeast artificial chromosome libraries in long-range mapp ing projects. To allow, general access to P1 clones, we have construct ed human and mouse P1 libraries. Clones have been picked into microtit er plates and used to prepare high-density filter grids, providing an efficient and easy screening system. Filters are being made available to other laboratories through the Reference Library System. In this wo rk, we have developed a reliable protocol for generating P1 clones, ba sed on the use of pulsed-field gel electrophoresis for size selection of DNA. A 1.2x genome coverage human library has been produced using t his method. A preliminary analysis of this library is described.