C. Pelegri et al., IMMUNOHISTOCHEMICAL CHANGES IN SYNOVIAL TISSUE DURING THE COURSE OF ADJUVANT ARTHRITIS, Journal of rheumatology, 22(1), 1995, pp. 124-132
Objective. To study immunohistochemical changes occurring in knee syno
vial membranes of rats during the time course of adjuvant arthritis (A
A). The effect of treatment with dexamethasone after establishment of
arthritis was also studied. Methods. AA was induced in Wistar rats by
means of a single injection of a suspension of Mycobacterium butyricum
. On Days 7, 14, 17, 21, 28 and 42 after induction, synovial membranes
were obtained, frozen and sectioned on a cryostat. Tissue sections we
re tested by peroxidase-antiperoxidase method, using the following mon
oclonal antibodies: OX19 (CD5), W3/25 (CD4), OX8 (CD8), OX6 (Ia), OX33
(LCA) and OX39 (CD25). Results. Knee synovial membranes obtained from
arthritic rats on days of maximum inflammation showed few or no CD5cells, a higher proportion of CD8+ cells, and a higher number of CD4and Ia+ cells than healthy synovial tissues. CD25+ cells were observed
from Day 7 postinduction and remained numerous throughout the study.
Conclusion. Few T lymphocytes (CD5+) were found in arthritic synovial
membrane, whereas an increase of cells expressing CD8, CD4, CD25, Ia,
and LCA was found. These increases (except CD8) are not evident in dex
amethasone treated rats.