PHOSPHOROTHIOATE OLIGONUCLEOTIDES - LARGELY REDUCED (N-1)-MER AND PHOSPHODIESTER CONTENT THROUGH THE USE OF DIMERIC PHOSPHORAMIDITE SYNTHONS

Authors
KROTZ AH KLOPCHIN P COLE DL RAVIKUMAR VT
Citation
Ah. Krotz et al., PHOSPHOROTHIOATE OLIGONUCLEOTIDES - LARGELY REDUCED (N-1)-MER AND PHOSPHODIESTER CONTENT THROUGH THE USE OF DIMERIC PHOSPHORAMIDITE SYNTHONS, Bioorganic & medicinal chemistry letters, 7(1), 1997, pp. 73-78
Citations number
25
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
7
Issue
1
Year of publication
1997
Pages
73 - 78
Database
ISI
SICI code
0960-894X(1997)7:1<73:PO-LR(>2.0.ZU;2-V
Abstract
Phosphorothioate oligonucleotides synthesized through an assembly of d imeric phosphoramidite synthons on controlled pore glass solid support show a significantly improved impurity profile compared to oligomers synthesized through a coupling of standard monomer phosphoramidites. A greater than 70% reduction of the (n-1)-mer population and a ca 50% r eduction of phosphodiester linkages has been achieved. Copyright (C) 1 996 Elsevier Science Ltd