Chicken anaemia virus (CAV) was isolated for the first time in Chile f
rom thymus samples obtained from SPF chickens experimentally infected
with CAV serologically positive tissues. Isolation was conducted in MD
CC-MSB1 cell cultures. Virus replication was evidenced by both the ind
uction of a cytopathologic effect characterized by enlarged swollen ce
lls and by the identification of the virus in the cells through immuno
fluorescence tests employing a specific reference monoclonal antibody.