S. Oiki et al., MG2-DEPENDENCE OF VOLUME-SENSITIVE CL- CHANNELS IN HUMAN EPITHELIAL-CELLS( AND ATP), Japanese Journal of Physiology, 44, 1994, pp. 77-79
During osmotic cell swelling whole-cell Cl- currents were recorded in
cultured human small intestinal epithelial cells (Intestine 407). When
intracellular ATP was depleted, swelling-induced Cl- currents were ab
olished. Increases in intracellular free ATP enhanced the Cl- current
in a concentration-dependent manner. The presence of intracellular fre
e Mg2+ ions suppressed the current. Non-hydrolyzable ATP analogs (AMP-
PNP and ATP gamma S), ADP, and GTP could substitute for ATP. Thus, it
is concluded that ATP plays an indispensable role in activation of the
volume-sensitive Cl- channel without hydrolysis and that intracellula
r Mg2+ is an additional regulator for volume-sensitive Cl- channels in
Intestine 407 cells.