A STAPHYLOCOCCUS-AUREUS AUTOLYSIN THAT HAS AN N-ACETYLMURAMOYL-L ALANINE AMIDASE DOMAIN AND AN ENDO-BETA-N-ACETYLGLUCOSAMINIDASE DOMAIN - CLONING, SEQUENCE-ANALYSIS, AND CHARACTERIZATION

Authors
OSHIDA T SUGAI M KOMATSUZAWA H HONG YM SUGINAKA H TOMASZ A
Citation
T. Oshida et al., A STAPHYLOCOCCUS-AUREUS AUTOLYSIN THAT HAS AN N-ACETYLMURAMOYL-L ALANINE AMIDASE DOMAIN AND AN ENDO-BETA-N-ACETYLGLUCOSAMINIDASE DOMAIN - CLONING, SEQUENCE-ANALYSIS, AND CHARACTERIZATION, Proceedings of the National Academy of Sciences of the United Statesof America, 92(1), 1995, pp. 285-289
Citations number
28
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
92
Issue
1
Year of publication
1995
Pages
285 - 289
Database
ISI
SICI code
0027-8424(1995)92:1<285:ASATHA>2.0.ZU;2-N
Abstract
The Tn551 insertion site of the autolysis-deficient Staphylococcus aur eus mutant RUSAL2 was cloned and used to identify the autolysis gene a tl in the parent strain, RN450. The open reading frame for atl was 376 8 bp in length, encoding a deduced protein of 1256 amino acids and mol ecular size of 137,381 Da. The atl gene product is a bifunctional prot ein that has an amidase domain and an endo-beta-N-acetylglucosaminidas e domain which must undergo proteolytic processing to generate the two extracellular lytic enzymes found in the culture broth of S. aureus.