The fluoresence of a nitrification/denitrification tank in an activate
d sludge nutrient removal process was monitored over a two month perio
d. The process employs an alternating mode of operation which subjects
the sludge to a periodically changing environment (aerobic, anoxic an
d anaerobic) at the point in the process where the fluoresence is meas
ured. The fluoresence signal decreased slowly during aerated periods a
nd increased slowly during anoxic (denitrifying) periods. An abrupt ri
se in the fluoresence signal was found to occur upon the transition fr
om anoxic to anaerobic conditions. The minimum fluoresence signal occu
rring during each operation cycle appeared to define a baseline whose
general upward and downward movements correlated well with peak oxygen
uptake rate,