Wl. Vannoort et al., PURIFICATION OF ISOTRANSFERRINS BY CONCANAVALIN-A SEPHAROSE CHROMATOGRAPHY AND PREPARATIVE ISOELECTRIC-FOCUSING, European journal of clinical chemistry and clinical biochemistry, 32(12), 1994, pp. 885-892
1. From pooled serum containing genetically homogeneous transferrin C-
1, transferrin was purified and separated in three fractions (tri-tri-
, bi-tri- and bi-bi-antennary transferrin C-1), using Concanavalin A-S
epharose. 2. Each of these fractions was separated into its sialic aci
d-dependent subfractions by preparative isoelectric focusing. Sixteen
iso-transferrin C-1 fractions were obtained, which differed in their d
egree of glycan branching and/or their sialic acid content. 3. Prelimi
nary carbohydrate analyses suggest that in some iso-transferrins the N
-acetylglucosamine and the galactose content is lower than expected.