THE ACCURACY OF CREATININE METHODS BASED ON THE JAFFE REACTION - A QUESTIONABLE MATTER

The determination of creatinine in serum based on the Jaffe reaction w as evaluated with four current analysers. In particular, the comparabi lity of results was determined also with survey specimens. Recalibrati on of 3 out of 4 modifications was necessary, based on the results of patient samples as verified with a HPLC-method. One of the methods pro ved to give an unacceptable scatter for the results in the lower range (30-150 mu mol/l). A limited interference study (haemoglobin, lipids, bilirubin and acetone) and a method assessment with quality control s era supported the conclusion that the overall accuracy of creatinine m ethods based on the Jaffe reaction is questionable.