DETECTION OF GIANT MYOFIBRILLAR PROTEINS CONNECTIN AND NEBULIN BY ELECTROPHORESIS IN 2-PERCENT POLYACRYLAMIDE SLAB GELS STRENGTHENED WITH AGAROSE

Authors
TATSUMI R HATTORI A
Citation
R. Tatsumi et A. Hattori, DETECTION OF GIANT MYOFIBRILLAR PROTEINS CONNECTIN AND NEBULIN BY ELECTROPHORESIS IN 2-PERCENT POLYACRYLAMIDE SLAB GELS STRENGTHENED WITH AGAROSE, Analytical biochemistry, 224(1), 1995, pp. 28-31
Citations number
11
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
224
Issue
1
Year of publication
1995
Pages
28 - 31
Database
ISI
SICI code
0003-2697(1995)224:1<28:DOGMPC>2.0.ZU;2-M
Abstract
We have established an improved method of sodium dodecyl sulfate-polya crylamide gel electrophoresis to facilitate analysis of giant myofibri llar proteins connectin and nebulin, whose molecular masses are about 3000 and 700 kDa, respectively. This method consists of 2% polyacrylam ide slab gels strengthened with agarose and a specific buffer system. Our 2% polyacrylamide slab gels are superior to usual 1.8 or 2% polyac rylamide disc gels in preparation, handling, and resolution. (C) 1995 Academic Press, Inc.