CHARACTERIZATION OF ANTIBODY-BINDING TO CELL-SURFACE ANTIGENS USING APLASMA MEMBRANE-BOUND PLATE ASSAY

A procedure has been developed for measuring antibody binding to cell surface antigens using an immobilized plasma membrane fraction. In thi s method, isolated plasma membranes are dried onto wells of a 96-well microtiter plate and incubated with antibodies that recognize a cell s urface protein. Bound antibody is detected indirectly using an enzyme- linked or fluorescently tagged second antibody. Alternatively, the pri mary antibody itself can be labeled and its binding can be detected di rectly. The assay is simple and fast and provides several advantages o ver whole cell binding assays currently in widespread use. (C) 1995 Ac ademic Press, Inc.