PEROXIDASE-MODIFIED AND TETRACYANOQUINODIMETHANE-MODIFIED GRAPHITE PASTE ELECTRODE FOR THE MEASUREMENT OF GLUCOSE-LACTATE GLUTAMATE USING ENZYME-PACKED BED REACTOR
Pc. Pandey et Hh. Weetall, PEROXIDASE-MODIFIED AND TETRACYANOQUINODIMETHANE-MODIFIED GRAPHITE PASTE ELECTRODE FOR THE MEASUREMENT OF GLUCOSE-LACTATE GLUTAMATE USING ENZYME-PACKED BED REACTOR, Analytical biochemistry, 224(1), 1995, pp. 428-433
A flow injection analysis sensor for the measurement of glucose/lactat
e/glutamate is reported. The glucose oxidase/glutamate oxidase/lactate
oxidase was immobilized on silanized controlled pore glass particles
and packed into a Teflon column (i.d., 1.2 mm; length, 40 mm) to give
a bed for glucose/lactate/glutamate. The hydrogen peroxide formed by t
he enzymatic reaction in the packed bed was monitored by a horseradish
peroxidase- and tetracyanoquinodimethane (TCNQ)- modified graphite pa
ste electrode at 50 mV vs Ag/AgCl. The glucose oxidase/lactate oxidase
/glutamate oxidase were regenerated in the packed bed, whereas peroxid
ase was regenerated in the TCNQ-mediated graphite paste electrode by t
he oxidation of TCNQ. The oxidized TCNQ was electrochemically reduced
at 50 mV vs Ag/AgCl. The cathodic current obtained by the reduction of
TCNQ determined the concentration of the injected analytes in the pac
ked bed. The system showed very rapid response. Response curves for th
e analysis of peroxide, glucose, lactate, and glutamate are reported.
(C) 1995 Academic Press, Inc.