PRESENCE OF NUCLEAR ANDROGEN RECEPTOR-LIKE IMMUNOREACTIVITY IN NEUROKININ B-CONTAINING NEURONS OF THE HYPOTHALAMIC ARCUATE NUCLEUS OF THE ADULT MALE-RAT
P. Ciofi et al., PRESENCE OF NUCLEAR ANDROGEN RECEPTOR-LIKE IMMUNOREACTIVITY IN NEUROKININ B-CONTAINING NEURONS OF THE HYPOTHALAMIC ARCUATE NUCLEUS OF THE ADULT MALE-RAT, Neuroscience letters, 182(2), 1994, pp. 193-196
Dual label immunofluorescence was used in the brain of normal, non col
chicine-treated, adult male rats in order to characterize the neurons
possessing the nuclear androgen receptor in the hypothalamic arcuate n
ucleus, The affinity purified PG21 rabbit serum to the rat androgen re
ceptor (AR) was used in conjunction with a polyclonal guinea pig antis
erum to peptide 2, a sequence within the precursor to neurokinin B (NK
B). All NKB-containing neurons were also immunoreactive for the AR, an
d made up a large proportion (about 60%) of the arcuate cells with det
ectable and specific AR immunoreactivity.