K. Yang et al., SUSTAINED EXPRESSION OF FUNCTIONAL NERVE GROWTH-FACTOR IN PRIMARY SEPTOHIPPOCAMPAL CELL-CULTURES BY LIPOSOME-MEDIATED GENE-TRANSFER, Neuroscience letters, 182(2), 1994, pp. 291-294
We examined liposome-mediated gene transfection of nerve growth factor
(NGF) in primary central nervous system cultures. RT-PCR analyses det
ected increased expression of NGF mRNA one day after liposome-mediated
NGF gene transfection. ELISA studies detected large increases in NGF
protein in cells and in culture medium after NGF gene transfection. Ce
lls continued to secrete NGF into the medium for at least 2 weeks. NGF
bioassays confirmed that the NGF secreted after gene transfection was
biologically active.