INFLUENCE OF PROSTAGLANDINS ON GLUCOSE-TRANSPORT IN ISOLATED RAT UTERUS

Glucose transport by uterine strips from ovariectomized estrogenized r ats was explored. Sugar transport was significantly different from sac arose values (non-specific diffusion) only after 60 min of incubation. The addition of cytochalasin B demonstrated that we are measuring a s pecific mechanism for glucose transport. Insulin-enhanced sugar transp ort only at 0.5 or 0.25 U/ml prostaglandin E(1) (PGE(1)), PGE(2) and P GF(2 alpha) (10(-7) M) significantly improved glucose transport, but i ndomethacin (10(-6) M) failed in modifying this parameter in either co ntrol nor insulin-treated tissues. We did not observe an additive or s ynergistic action between PGE(2) (10(-7) M) and insulin (used at maxim al or submaximal concentration).